Dr. Raquibul Hasan

Associate Professor of Pharmaceutical Sciences

Raquibul HasanEducation

Research Background and Interests

My research laboratory studies the molecular pharmacology of ion channels and G-protein-coupled receptors (GPCRs) to identify new drug molecules and/or repurpose existing drugs to improve vascular dysfunction in cardiovascular diseases, including hypertension, heart disease, erectile dysfunction, and stroke. We offer both basic science projects and drug discovery projects for students, volunteers and trainees. Our basic science project studies physiological functions and pathological alterations of vascular ion channels, GPCRs and associated signaling pathways. Our drug discovery project involves in-silico screening of candidate drugs as well as repurposing of existing drugs followed by functional, biochemical and molecular studies. We perform our experiments on fresh isolated intact arteries and isolated smooth muscle and endothelial cells. Techniques used include pressurized arterial myography, simultaneous arterial calcium imaging and contractility measurement, in-vitro assays (e.g. nitric oxide/cGMP/cAMP measurement and enzyme activity assays) protein biochemistry (e.g. Western blotting, immunoprecipitation, biotinylation), mass-spectrometry, cell and molecular biology (e.g. PCR, DNA amplification, isolation and purification, point mutation, confocal imaging, cell culture and transfection), and RNAi for gene knockdown. We use genetic models of hypertension such as Spontaneously Hypertensive Rat (SHR) and the Stroke Prone Spontaneously Hypertensive Rat (SHRSP), as well as induced models of hypertension ( e.g. angiotensin II, salt and L-NAME) to address our research questions. Queries for potential training opportunities in my laboratory should be directed to hasan_r@mercer.edu.

News

Pharmacy professor working on first-of-its-kind drug for high blood pressureThe Den, 8/1/23

Pharmacy Researcher Receives NIH Grant to Study Novel Blood Vessel Targets – The Den, 9/9/21

Courses

  • PHA 329 Pharmacy Fundamentals
  • PHA 463 Cardiovascular and Renal Pharmacotherapy I
  • PHA 464 Cardiovascular and Renal Pharmacotherapy II
  • PHA 537 Gastrointestinal and Musculoskeletal Pharmacotherapy
  • PHA 547 Nervous System Pharmacotherapy
  • PHA 799 Thesis Research
  • PHA 899 Doctoral Research

Selected Publications

  • PubMed
  • Google Scholar
  • Hasan R, Leo MD, Muralidharan P, Mata-Daboin A, Yin W, Bulley S, Fernandez-Pena C, Mackay C, Jaggar JH. 2019. SUMO1-modificaton of PKD2 channels regulates arterial contractility. Proceedings of the National Academy of Sciences of the USA (PNAS). 116 (52) 27095-27104
  • Hasan R*, Lasker S, Hasan A, Parvez F, Zerin F, Zamila M, Rahman MIU, Akter S, Rahman MM, Subhan N, Alam MA. 2020. Canagliflozin attenuates isoprenaline-induced cardiac oxidative stress by stimulating multiple antioxidant and anti-inflammatory signaling pathways. Scientific Reports. 10:14459 * corresponding author
  • Hasan R*, Lasker S, Hasan A, Parvez F, Zerin F, Zamila M, Rahman MIU, Akter S, Rahman MM, Subhan N, Alam MA. 2020. Canagliflozin ameliorates renal oxidative stress and inflammation by stimulating AMPK-Akt-eNOS pathway in isoprenaline-induced oxidative stress model. Scientific Reports. 10:14659 * corresponding author
  • Mackay CE, Leo MD, Fernández-Peña C, Hasan R, Yin W, Mata-Daboin A, Bulley S, Gammons J, Mancarella S, Jaggar JH. 2020. Intravascular flow stimulates PKD2 channels in endothelial cells to reduce blood pressure. e-Life. 9:e56655
  • Mamun F, Zamila M, Rahman M, Subhan N, Hossain H, Hasan R, Alam MA, Haque MA. 2019. Polyphenolic compounds of litchi leaf augment kidney and heart functions in 2K1C rats, Journal of Functional Food
  • Hasan R and Zhang X. 2018. Ca2+ regulation of TRP Ion Channels. International Journal of  Molecular Sciences. 19(4):1256
  • Bulley S, Fernandez-Pena C, Hasan R, Leo MD, Muralidharan P, Mackay C, Evanson KW, Moreira-Junior L, Mata-Daboin A, Burris SK, Mackay CE, Wang Q, Kuruvilla KP, Jaggar JH. 2018. Arterial smooth muscle cell PKD2 (TRPP1) channels control systemic blood pressure. e-Life. 7:e42628
  • Hasan R, Leeson-Payne ATS, Jaggar JH, Zhang X. 2017. Calmodulin is responsible for Ca2+-dependent regulation of TRPA1 Channels. Scientific Reports. 7:45098
  • Li L, Hasan R, Zhang X. 2014. The basal thermal sensitivity of TRPV1 ion channel is determined by PKCβII. J. Neuroscience. 34(24): 8246-58. Equal contribution
  • Than JY, Li L, Hasan R, Zhang X. 2013. The excitation and modulation of TRPA1-, TRPV1- and TRPM8-expressing sensory neurons by the pruritogen chloroquine. Journal of Biological Chemistry. 288(18): 12818-27

Contact Dr. Hasan


Moye Pharmacy and Health Sciences Center #267
(678) 547-6223
hasan_r@mercer.edu